ABSTRACT
This study investigated the presence of the Treponema species in longstanding endodontic retreatment-resistant lesions of teeth with apical periodontitis, the association of this species with clinical/radiographic features, and the association among the different target species. Microbial samples of apical lesions were collected from twenty-five adult patients referred to endodontic surgery after unsuccessful root canal retreatment. Nested-PCR and conventional PCR were used for Treponema detection. Twenty-three periradicular tissue samples showed detectable levels of bacterial DNA. Treponema species were detected in 28% (7/25) of the cases. The most frequently detected species were T. socranskii (6/25), followed by T. maltophilum (3/25), T. amylovorum (3/25), T. lecithinolyticum(3/25), T. denticola (3/25), T. pectinovorum (2/25) and T. medium(2/25). T. vicentii was not detected in any sample. Positive statistical association was found between T. socranskiiand T. denticola,and between T. maltophilumand T. lecithinolyticum. No association was detected between the presence of any target microorganism and the clinical or radiographic features. Treponemaspp.are present, in a low percentage, in longstanding apical lesions from teeth with endodontic retreatment failure.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Periapical Periodontitis/microbiology , Periapical Periodontitis/therapy , Periapical Tissue/microbiology , Treponema/isolation & purification , Treponemal Infections/microbiology , Chi-Square Distribution , DNA, Bacterial/analysis , Polymerase Chain Reaction , Periapical Periodontitis/pathology , Periapical Periodontitis , Periapical Tissue/pathology , Periapical Tissue , Retreatment , Root Canal Therapy , Treatment Failure , Treponemal InfectionsABSTRACT
Introduction: The aim of this study was to evaluate the quality and bond strength of three root filling techniques (lateral compaction, continuous wave of condensation and Tagger’s Hybrid technique [THT]) using micro‑computed tomography (CT) images and push‑out tests, respectively. Materials and Methods: Thirty mandibular incisors were prepared using the same protocol and randomly divided into three groups (n = 10): Lateral condensation technique (LCT), continuous wave of condensation technique (CWCT), and THT. All specimens were filled with Gutta‑percha (GP) cones and AH Plus sealer. Five specimens of each group were randomly chosen for micro‑CT analysis and all of them were sectioned into 1 mm slices and subjected to push‑out tests. Results: Micro‑CT analysis revealed less empty spaces when GP was heated within the root canals in CWCT and THT when compared to LCT. Push‑out tests showed that LCT and THT had a significantly higher displacement resistance (P < 0.05) when compared to the CWCT. Bond strength was lower in apical and middle thirds than in the coronal thirds. Conclusions: It can be concluded that LCT and THT were associated with higher bond strengths to intraradicular dentine than CWCT. However, LCT was associated with more empty voids than the other techniques.
Subject(s)
Dental Bonding/methods , Endodontics/methods , Root Canal Therapy/methods , Tomography, X-Ray ComputedABSTRACT
A calcium aluminate-based endodontic material, EndoBinder, has been developed in order to reduce MTA negative characteristics, preserving its biological properties and clinical applications. Objectives: The aim of this study was to evaluate the cytotoxicity, antimicrobial activity, pH, solubility and water sorption of EndoBinder and to compare them with those of white MTA (WMTA). Material and Methods: Cytotoxicity was assessed through a multiparametric analysis employing 3T3 cells. Antimicrobial activity against Enterococcus faecalis (ATCC 29212), Staphylococcus aureus. (ATCC 25923) and Candida albicans (ATCC 10556) was determined by the agar diffusion method. pH was measured at periods of 3, 24, 72 and 168 hours. Solubility and water sorption evaluation were performed following ISO requirements. Data were statistically analyzed by ANOVA and Tukey`s test with a significance level of 5%. Results: EndoBinder and WMTA were non-cytotoxic in all tested periods and with the different cell viability parameters. There was no statistical differences between both materials (P>.05). All tested materials were inhibitory by direct contact against all microbial strains tested. EndoBinder and WMTA presented alkaline pH in all tested times with higher values of pH for WMTA (P<.05). Both materials showed values complying with the solubility minimum requirements. However, EndoBinder showed lower solubility than WMTA (P<.05). No statistical differences were observed regarding water sorption (P>.05). Conclusion: Under these experimental conditions, we concluded that the calcium aluminate-based endodontic material EndoBinder demonstrated suitable biological and physicochemical properties, so it can be suggested as a material of choice in root resorption, perforations and root-end filling. .